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Solvent extraction, GC-MS for 13 phthalate esters
MatTek EpiDerm™ reconstructed human epidermis model
Ethylene glycol by GC-FID after extraction
Simulated use extraction, GC-FID headspace analysis
Additional extraction cycle for EO/ECH
Method validation with count standards at 10µm and 25µm
Extraction in particle-free water, light obscuration counting
Cold water extraction, pH measurement
Additional sample pH test
Direct/extract contact with RBCs, hemoglobin release
Mouse systemic injection, polar/non-polar extracts
Mouse systemic toxicity per USP Class VI requirements
Rabbit intracutaneous reactivity test with extracts
Rabbit muscle implantation, histopathology evaluation
ATR-FTIR spectrum comparison with reference
Hexane extraction, LC-MS analysis
Water extraction, LC-MS screening for non-volatiles
IPA extraction, LC-MS non-volatile screening
Acid digestion, ICP-MS for heavy metals